The mouse monoclonal antibody clone 6H6 binds to human CD123, a 70-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily, also known as the Interleukin-3 receptor alpha chain. CD123 alone has a low affinity to IL-3 and develops a high affinity when associated with βc (common chain). The CD123 molecule is expressed predominantly on hematopoietic progenitor cells, macrophages, dendritic cells, basophils, eosinophils, monocytes, megakaryocytes, and some B cells. It is also present in myeloid, lymphoid, and vascular endothelial cells.

PRODUCT DETAILS

Clone: 6H6

Specificity: CD123

Reactivity: Human, Rhesus Macaque

Format: cFluor^® V670

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Sun Q, et al. 1996. Blood. 87:83-92.
2. Miyajima A. 1997. Leucocyte typing VI.854-855.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® V670 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® V670 is StarBright^™ Violet 670 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.