The 3B2/TA8 monoclonal antibody reacts with human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas, and absent on some B cell lines, fetal B cells, eosinophils, and granulocytes. CD99 is involved in spontaneous erythrocyte rosette formation may be involved in other T-cell and hematopoietic cell adhesion pathways.

PRODUCT DETAILS

Clone:        3B2/TA8

Reactivity:    Human

Format:        cFluor^® V610

Isotype:              Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Gelin C, et al. 1989. EMBO. 8:3253-3259.
2. Goodfellow PJ, et al. 1986. Science 234:740-743.
3. Pettersen RD, et al. 2001. J. Immunol. 166:4931-4942.

For Research Use Only. Not intended for use in diagnostic procedures.