cFluor® V570 anti-Mouse CD19 (6D5)
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説明
The 6D5 antibody reacts with mouse CD19, a 95-kDa glycoprotein which acts as a co-receptor, along with CD21 and CD81, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, throughout development, and is finally lost upon transition to mature plasma cells. CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as rat anti-mouse CD19 antibody clone ID3 in cross-competition assays. The 1D3 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: 6D5
Reactivity: Mouse
Format: cFluor® V570
Isotype: Rat IgG2a
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® V570 anti-Mouse CD19 (6D5), (right) vs. Isotype Control (left). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® V570 is StarBright™ Violet 570 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.