The mouse monoclonal antibody clone 3B2/TA8 binds to human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas and is absent on some B cell lines, fetal B cells, eosinophils, and granulocytes. CD99 is involved in spontaneous erythrocyte rosette formation and may be involved in other T cell and hematopoietic cell adhesion pathways.

PRODUCT DETAILS

Clone: 3B2/TA8

Specificity: CD99

Reactivity: Human

Format: cFluor^® V547

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Waclavicek, M., et al. 1998. J. Immunol 161:4671-8.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® V547 is equivalent to CF^®405L manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.