The UCHT1 monoclonal antibody binds to the human epsilon chain of the CD3 antigen/T cell antigen receptor, a 20- to 30-kDa protein complex. This complex contains a CD3γ, a CD3δ, a CD3ζ (CD247), two CD3ε, and a T cell receptor (α/β or γ/δ) heterodimer (Dong, D., et al. 1981. Nature. 573, 546–552). CD3 is found on all mature T cells, NKT cells, and thymocytes. It plays a role in recognizing antigen, activating cytotoxic T cells and T helper cells, and signal transduction (Weiss A, et al. 1991. Semin Immunol. (5):313-24).
The antibody was purified by affinity chromatography and conjugated to the fluorophore.

PRODUCT DETAILS

Clone: UCHT1

Reactivity: Human

Format: cFluor^® V475

Isotype: Mouse IgG1

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze.

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS SDS

EXAMPLE DATA

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For Research Use Only. Not intended for use in diagnostic procedures.

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