The HI9a monoclonal antibody binds to human CD9, a 24-kDa type III transmembrane glycoprotein. CD9, also known as tetraspanin, MRP-1, and DRAP-24, is a member of the tetraspanin family that is expressed on platelets, endothelial and epithelial cells and all major subsets of leukocytes including B cells, CD4+ T cells, CD8+T cells, NK cells, granulocytes, monocytes, macrophage⁽¹⁾. CD9 plays a role in diverse cellular processes including cellular adhesion, migration, platelet aggregation and B cell development ⁽²⁾⁽³⁾. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:        HI9a

Reactivity:    Human, African Green, Baboon, Cattle (Bovine, Cow), Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Rhesus, Sheep (Ovine)

Format:        cFluor^® V450

Isotype:              Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Reyes, Raquel et al. 2018. Frontiers in immunology vol. 9 863. 30
2. Machado-Pineda, Yesenia et al. 2018. Frontiers in immunology vol. 9 2474
3. Masellis-Smith A, et al. 1994. J Immunol. 152(6):2768-77

For Research Use Only. Not for use in diagnostic or therapeutic procedures.