The M5E2 monoclonal antibody binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS)¹. It is found that CD14 is a receptor for and binds to complexes of LPS and LBP with high affinity². It expresses on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells and macrophages, reticular dendritic cells and Langerhans cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  M5E2

Reactivity:          Human, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cow, Cynomolgus, Dog, Pigtailed Macaque, Rhesus, Squirrel Monkey

Format:                cFluor® V450

Isotype:               Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Pugin J, et al. 1998. Infect Immun. 66:1174
2. Wright SD, et al. 1990. Science. 249:1431

These products are for research use only. Not intended for use in diagnostic procedure.