The mouse monoclonal antibody clone 5E10C7 reacts with human CD66c, also known as CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6) and formerly termed Nonspecific Cross-reacting Antigen (NCA-90). CD66c is a ~90 kDa GPI-anchored member of the carcinoembryonic antigen (CEA) family within the immunoglobulin superfamily. It lacks transmembrane and intracellular domains and signals indirectly through lateral interactions with membrane partners. CD66c is constitutively expressed on neutrophils, monocytes, and columnar epithelial cells of the colon, and can be induced on T cells, B cells, and CD16-negative NK cells. On neutrophils, CD66c participates in homophilic and heterophilic adhesion with other CEACAM family members including CEACAM1, CEACAM5, and CEACAM8, and engagement of CD66c promotes upregulation of CD11b/CD18 and increased neutrophil adhesion to endothelial cells. CD66c is overexpressed in a broad range of tumor types including colorectal, pancreatic, lung, and breast carcinomas, and elevated expression has been associated with poorer prognosis and disease-free survival. CD66c expression in leukemic cells has also been correlated with BCR/ABL1 rearrangement. This antibody is reported for use in flow cytometric analysis.

PRODUCT DETAILS

Clone: 5E10C7

Specificity: CD66c

Reactivity: Human

Format: cFluor^® V435

Isotype: Mouse IgG1

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Skubitz, K.M. et al. 2008. J. Transl. Med. 6: 78.
2. Blumenthal, R.D. et al. 2007. BMC Cancer 7: 2.
3. Zheng, C. et al. 2024. Discov. Oncol. 15: 176.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® V435 is StarBright^™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.