The mouse monoclonal antibody clone L17F12 binds to human CD5, a 67 kDa single chain type I glycoprotein in the scavenger receptor family. CD5, also known as Leu-1, Ly-1, or T1, is expressed on thymocytes, T cells, and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction.

PRODUCT DETAILS

Clone: L17F12

Specificity: CD5

Reactivity: Human

Format: cFluor^® V435

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Challagundla P, Jorgensen JL, Kanagal-Shamanna R, et al. Archives of Pathology & Laboratory Medicine. 2014;138(7):903–909.
2. Friedman D, Mehtani DP, Vidler JB, Patten PEM, Hoogeboom R. bioRxiv 2023.12.27.573410
3. Kaplan D, Smith D, Meyerson, H. Proc. Natl. Acad. Sci. U.S.A. 2001;98(24):13850-13853.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® V435 is StarBright^™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.