The 3G8 monoclonal antibody binds to IgG receptor III (FcγRIII) that are in two forms: CD16a ((FcγRIIIA) and CD16b ((FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kD polypeptide-anchored transmembrane protein and a 48 kD GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages while CD16b is expressed on neutrophils^1,2. CD16a also plays a crucial role for antibody-dependent cellular cytotoxicity (ADCC) by NK cells³. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:              3G8

Reactivity:       Human

Format:            cFluor^® V420

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Wirthmueller U, et al. 1992. J Exp Med. 175:1381

2. Smed-Sörensen A, et al. 2008. Blood. 111:5037

3. Wei H Y, et al, 2016. Sci Rep. 6:34310

These products are for research use only. Not intended for use in diagnostic procedures.