The mouse monoclonal antibody clone CD25-4E3 binds to human CD25 (IL-2 receptor alpha, IL-2Rα) is a 55 kDa surface protein that serves as the low-affinity receptor for interleukin-2 (IL-2). While CD25 can bind IL-2 alone without inducing signaling, it is primarily found as part of the high-affinity IL-2 receptor complex, along with CD122 (IL-2 receptor beta) and CD132 (common gamma chain). CD25 is expressed during T and B cell development, on activated T and B cells, macrophages, and at high levels on natural regulatory T cells (Tregs) in the thymus and periphery, as well as on induced peripheral Tregs. IL-2 receptor signaling is involved in lymphocyte proliferation, survival, and differentiation, influencing Th17 and Treg cell function. CD25 also plays a role in dendritic cell activity and is a marker for Hodgkin’s disease and autoimmune conditions such as Type I diabetes.

PRODUCT DETAILS

Clone: CD25-4E3

Specificity: CD25

Reactivity: Human

Format: cFluor^® UV570

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® UV570 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® UV570 is StarBright^™ UltraViolet 575 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.