The mouse monoclonal antibody clone HI98 binds to human CD15, a 220-kDa carbohydrate structure. CD15 is also known as 3-fucosyl-N-acetyllactosamine (3-FAL), Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is highly expressed on granulocytes, including neutrophils, eosinophils, and some on monocytes. It has also been shown to be expressed on Langerhans cells. It is absent from lymphocytes or basophils. CD15, a marker for human myeloid cells, is involved in various cell functions, including phagocytosis, bacterial activity, neutrophil adhesion to dendritic cells, and chemotaxis.

PRODUCT DETAILS

Clone: HI98

Specificity: CD15

Reactivity: Human, Chimpanzee

Format: cFluor^® UV515

Isotype: Mouse IgM, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® UV515 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® UV515 is StarBright^™ UltraViolet 510 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.