The mouse monoclonal antibody clone WM15 binds to human CD13, a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin, including granulocytes, monocytes, mast cells, and GM-progenitor cells. The majority of acute myeloid leukemia (AML) cases and chronic myeloid leukemia (CML) cases—particularly during the myeloid blast crisis phase—express this marker, whereas it is present in only a smaller proportion of lymphoid leukemias. CD13 is also present on fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is absent from normal lymphocytes, platelets, and erythrocytes. CD13 is reported to have many different functions, including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. CD13 is also reported to serve as a receptor for some strains of human coronaviruses (HCV).

PRODUCT DETAILS

Clone: WM15

Specificity: CD13

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque

Format: cFluor^® UV395

Isotype: Mouse IgG1

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Favaloro EJ, et al. 1987. Dis Markers. 5(4):215-225.
2. Nakamura Y, et al. 1994. Blood. 83(5):1442-3.
3. Freeman SD, et al. 1995. Blood. 85(8):2005-2012.
4. Walter RB, et al. 2007. Traffic. 9(2):267-279.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® UV395 is StarBright^™ UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.