The mouse monoclonal antibody clone W6D3 binds to human CD15, a 220-kDa carbohydrate structure. CD15 is also known as 3-fucosyl-N-acetyllactosamine (3-FAL), Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is highly expressed on granulocytes, including neutrophils, eosinophils, and some on monocytes. It has also been shown to be expressed on Langerhans cells. It is absent from lymphocytes or basophils. CD15, a marker for human myeloid cells, is involved in various cell functions, including phagocytosis, bacterial activity, neutrophil adhesion to dendritic cells, and chemotaxis.

PRODUCT DETAILS

Clone: W6D3

Specificity: CD15

Reactivity: Human, Cynomolgus, Bovine

Format: cFluor^® R840^1,2

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Briddell RA, et al, 1992. Blood. 79:3159-3167.
2. Broudy, V.C. et al. 1998.Blood. 91 (3): 898-906.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® R840 is equivalent to APC-CF^® 790T manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

²cFluor^® R840 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives