The 2D1 monoclonal antibody binds to isoforms of human CD45, a 180-240-kDa type I membrane glycoprotein, also known as LCA (leukocyte common antigen). CD45 is the first and prototypic receptor-like protein tyrosine phosphatase that expresses on all human leukocytes^1,2. It is absent on mature erythrocytes, platelets, and non-hematopoietic cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  2D1

Reactivity:          Human

Format:                cFluor^® R780¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Terry LA, et al. 1990. Immunology. 64:331
2. Hermiston ML, et al. 2003. Annu Rev Immunol. 21:107

These products are for research use only. Not intended for use in diagnostic procedures.

¹cFluor^® R780 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.