The RPA-2.10 monoclonal antibody binds to human CD2, a 50-kDa type I transmembrane glycoprotein ⁽¹⁾⁽²⁾.  CD2, also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R), is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion ⁽³⁾. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  RPA-2.10

Reactivity:          Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Swine (Pig, Porcine)

Format:                cFluor^® R720*

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Moingeon P, et al. 1989. Immunol Rev. Oct; 111: 111-44
2. Schraven B. J Biol Regul Homeost Agents. 2000 Jul-Sep;14(3):223-5
3. Binder C, et al. Front Immunol. 2020 Jun 9; 11: 1090

These products are for research use only. Not intended for use in diagnostic procedures.

*cFluor® R720 is equivalent to CF®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.