The CD7-6B7 monoclonal antibody binds to human CD7, a 40-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD7, also known as GP40, is found on T cells, NK cells, thymocytes, and pre-B cells1. CD7 is called T cell leukemia antigen for its expression on acute lymphocytic leukemia and some acute myeloid leukemia2. Studies show that cross-linking of CD7 significantly increases CD7- associated PI3-kinase activity, suggesting the antigen’s functional role in inducing calcium flux in T lymphocytes, and T and NK cell activation3. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:          CD7-6B7

Reactivity:       Human

Format:        cFluor^® R659

Isotype:              Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1.  Rabinowich H, 1994. J Immunol. 152(2):517-526.
2. Aruffo A, et al. 1987. EMBO J. 6(11):3313-3316.
3. Lee DM, et al. 1996. Int Immunol. 8(8):1195-1203

These products are for research use only. Not intended for use in diagnostic procedures.