The Cytek cFluor Immunoprofiling Kit allows for the identification of helper T cells, cytotoxic T cells, B cells, NK cells and monocytes in human peripheral blood mononuclear cells and in whole blood. The reagents in this kit help to distinguish different subsets of T, B and NK cells; including regulatory T cells, naïve T cells, activated T cells, memory T cells, effector T cells, naïve B cell, memory B cells, nonclassical and classical monocytes. 

PRODUCT DETAILS

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. (Do not freeze)

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA.

Data Sheets:       TDS SDS

EXAMPLE DATA 

RECOMMENDED USAGE

Human peripheral mononuclear cells (PBMC) or whole blood have been tested to validate the performance of this kit. 

• Recommended Staining Protocol
• Download SpectroFlo Experiment Template
• Download cFluor Fluorescent Tag List for SpectroFlo
• Download SpectroFlo Acquisition Protocol
• Download the FCS Express Template
• Download the cFluor® 14 Color Immunoprofiling™ Kit Expansion Guide

Cytek dye cFluor R780 and its conjugates were developed, manufactured and commercialized by BioLegend, Inc. under the trademark APC/Fire750™.

Cytek dye cFluor R840 and its conjugates were developed, manufactured and commercialized by BioLegend, Inc. under the trademark APC/Fire810™.

Fluor conjugated antibody manufactured and supplied by BioLegend Inc.

cFluor™ V547, cFluor™ B515, cFluor™ R668 and cFluor™ R720 are equivalent to CF®405L, CF®488A, CF®647 and CF®700 respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. 

These products are for research use only and are not intended for diagnostic use.