The Cytek^® cFluor^® Immunoprofiling Kit, 14 Color allows for the identification of helper T cells, cytotoxic T cells, B cells, NK cells, and monocytes in human peripheral blood mononuclear cells and in whole blood. The reagents in this kit help to distinguish different subsets of T, B, and NK cells; including regulatory T cells, naïve T cells, activated T cells, memory T cells, effector T cells, naïve B cell, memory B cells, nonclassical, and classical monocytes.

• Download Brochure

Products required for the panel but not included:

• ViaDye^™ Red Fixable Viability Dye Kit

PRODUCT DETAILS

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from Light. Do not freeze

Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA.

Data Sheets: TDS | SDS

The panel contains the following single vial reagents.

Table 1: Composition of the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color (P/N R7-40000). ViaDye^™ Red Fixable Viability Dye Kit (P/N R7-60008) is available to purchase separately.

DATA 

Figure 1: Results of healthy donor PBMCs stained with the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color. A) Identification of monocytes with CD14 and CD16. B) CD3 was used to gate T-cells. C) From the T-cell population, CD8 and CD56 were used to obtain the NKT-like population. D) CD4 and CD8 T-cell populations. E) Characterization of memory and effector CD8 T-cells. F) Characterization of memory and effector CD4 T-cells and identification of regulatory T-cells. G) The CD3^- population further divided into CD19⁺ and CD56⁺ subsets. H) NK cell populations. I) Characterization of naïve and memory B-cells.

RECOMMENDED USAGE

Human peripheral mononuclear cells (PBMC) or whole blood has been tested to validate the performance of this kit. Please refer to the section below to download this kit's staining protocols, data acquisition protocols, and experiment templates.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

• Download the Sample Preparation Guidelines for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color
• Download the Acquisition Protocol for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color
• Download the SpectroFlo^® Experiment Template for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color
• Download the FCS Express Template for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color
• Download the Assay Tags for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color
• Download the Expansion Guide for the Cytek^® cFluor^® Immunoprofiling Kit, 14 Color

REFERENCES

Clevers H et al. Ann Rev Immunol. 1988 6, 629 (1988)
Evans RL et al. Immunol. 78, 544 (1981)
Cole DK et al. Cell Mol Immunol. 1, 81 (2004)
Ferrero E et al. J. Immunol. 145, 331 (1990)
Wirthmueller U et al. J Exp Med. 175, (1992)
Dennis O et al. J Immunol. 170, 73 (2003)
Shevach E. Nat Rev Immunol. 2, 389 (2002)
Vossen M et al. J Immunol. 180, 3739 (2008)
Fritsch R et al. J Immunol. 175, 6489 (2005)
Loken MR et al. Cytometry. 11, 453 (1990)
Kumar, Connors & Farber. Immunity. 48, 202 (2018)
Poli A et al. Immunology. 126, 458 (2009)
Carrette, F & Surh, C. Semin Immunol. 24, 209 (2012)
Mahnke Y et al. Eur J Immunol. 43, 2797 (2013)
Goodwin R et al. Cell. 73, 447 (1993)

For Research Use Only. Not intended for use in diagnostic procedures.

cFluor^® BYG667, cFluor^® BYG710 and cFluor^® BYG781 are tandem dyes made with R-PE. cFluor^® B690 is a tandem dye made with PerCP. cFluor^® R780 and cFluor^® R840 are tandem dyes made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

cFluor^® B515, cFluor^® R668, cFluor^® R720, cFluor^® R840, and cFluor^® V547 are equivalent to CF^®488A, CF^®647, CF^®700, APC-CF^®790T, and CF^®405L respectively, manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.