The mouse monoclonal antibody clone HM47 binds to human CD79a, an intracellular component of the B-cell receptor (BCR) complex. CD79a (also known as mb-1 or Igα) is a 47 kDa type I transmembrane signaling molecule expressed in the cytoplasm and membrane of B-lineage cells from the earliest recognizable B-cell precursors through mature B cells, and is retained in most B-cell neoplasms. CD79a forms a heterodimer with CD79b to mediate surface immunoglobulin (sIg) transport and signal transduction via ITAM-dependent pathways. Clone HM47 recognizes an intracellular epitope of CD79a and is suitable for flow cytometry following appropriate fixation/permeabilization.

PRODUCT DETAILS

Clone: HM47

Specificity: CD79a

Reactivity: Human, Mouse

Format: cFluor^® BYG781

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Mason DY, Cordell JL, Brown MH, et al. 1995. J Pathol. 175:151–160.
2. van Dongen JJM, Lhermitte L, Böttcher S, et al. 2012. Cytometry A. 81A:614–636.
3. Bene MC, Nebe T, Bettelheim P, et al. 1995. Leukemia. 9:1783–1786.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG781 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.