The mouse monoclonal antibody clone 3.9 binds to human CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin α-receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g., fibrinogen (cell-matrix). In addition, integrin α's are active signaling receptors that recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin α-receptors consist of distinct combinations of integrin α-chains, which are differentially expressed. Integrin alpha X (CD11c) assembles with integrin beta-2 (CD18) into a receptor known as CR4, which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells. CD11c is prominently expressed on tissue macrophages and detected on activated neutrophils, granulocytes, some types of activated T cells, and intestinal intraepithelial lymphocytes (IEL).

PRODUCT DETAILS

Clone: 3.9

Specificity: CD11c

Reactivity: Human

Format: cFluor^® BYG781

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG781 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.