The CEW33D monoclonal antibody binds to human CD183, also known as CXCR3. CD183 is a G protein-coupled chemokine receptor that interacts with ligands CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). CD183 is strongly associated with type 1 immunity. It is induced in naive T cells upon activation and remains upregulated in T helper type (Th)1 cells, CD8 effector cells, NK cells and NKT cells. CD183-ligand interactions mediate infiltration of inflamed tissues in normal type 1 immune responses as well as in many inflammatory and autoimmune diseases. CD183 is also expressed on some B cells and plasmacytoid DC.

The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone: CEW33D

Specificity: CD183 (CXCR3)

Reactivity: Human

Format: cFluor^® BYG750¹

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. PBMCs may require a lower volume for optimal staining when used in combination with other reagents. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Loetscher M, et al. 1996. J. Exp. Med. 184:963-969.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009-2021.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909-L918.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887-899.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089-1096.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67-78.
7. Hoerning A, et al. 2011. Eur J Immunol 41:2291-2302.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.