The B1 monoclonal antibody binds to human γδ T cell receptor (TCR γδ). γδ TCR is a T cell receptor consisting of two glycoprotein chains, a 45-60 kDa gamma chain and a 40-60 kDa delta chain. Unlike conventional αβ T cell receptor, which recognizes peptide bound to MHC, γδ TCR recognizes antigen in non-MHC restricted way^1,2. TCR γδ associates with CD3 to play a role in signal transduction and is found on thymocytes and subset of T cells. Some studies suggest its role in antigen presentation and autoimmune disease³.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS 

Clone: B1

Reactivity: Human, African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus

Format: cFluor^® BYG710¹

Isotype: Mouse IgG1, κ

Tested Dilution: 5 μL / test

Application:  Flow Cytometry

Storage:  2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets: TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Liu Y, et al. 2020. Cells. 13;9(5):1206
2. Kabelitz D, et al. 2000. Int Arch Allergy Immunol. 122(1):1-7
3. Bonneville M, et al. 2010, Nat Rev Immunol. 10(7):467-478

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

¹cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.