The mouse monoclonal antibody clone 5E10 reacts with human CD90, also known as Thy-1 (thymus cell antigen-1), a 25–37 kDa GPI-anchored glycoprotein and the smallest member of the immunoglobulin superfamily. CD90 contains a single V-type Ig domain and, lacking an intracellular domain, signals through lateral interactions with Src family kinases, G-inhibitory proteins, and RGD-binding integrins within lipid rafts. CD90 is expressed on thymocytes, neurons, fibroblasts, activated endothelial cells, and a small subset of CD3+CD4+ T cells in peripheral blood. In the hematopoietic compartment, CD90 marks a subset of CD34+ prothymocytes and hematopoietic stem cells in bone marrow, fetal liver, and cord blood, where the CD90+CD34+ population is enriched for long-term culture-initiating cells. CD90 is also a defining surface marker of mesenchymal stromal cells (MSCs), included in the minimal criteria established by the International Society for Cell Therapy. CD90 participates in cell-cell and cell-matrix adhesion, T cell signal transduction, neurite outgrowth, and regulation of MSC differentiation capacity. This antibody is reported for use in flow cytometric analysis, immunoprecipitation, western blotting, and immunohistology of frozen tissue sections.

PRODUCT DETAILS

Clone: 5E10

Specificity: CD90

Reactivity: Human

Format: cFluor^® BYG710

Isotype: Mouse IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Dominici, M. et al. 2006. Cytotherapy 8: 315–317.
2. Rege, T.A. and Hagood, J.S. 2006. Am. J. Respir. Cell Mol. Biol. 34: 646–655. Sauzay, C. et al. 2019. Front. Cell Dev. Biol. 7: 66.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.