The SK1 monoclonal antibody binds to human CD8a, a 32-34-kDa type I glycoprotein that forms either as an α/α homodimer α/β heterodimer with its isoform CD8b. CD8 is found on thymocytes, a subset of peripheral blood T cells, majority expressing α/β heterodimer, and NK cells, majority expressing α/α homodimer1. CD8 plays a role in antigen recognition and class I MHC-restricted T cell development. CD8 functions are associated with two domains of CD8a. CD8a ectodomain binding to the α3 domain of MHC class I increases the adhesion between CD8+ T lymphocytes and target cells. The cytoplasmic tail binding to the tyrosine kinase p56lck contributes to signal transduction and T cell activation2,3. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  SK1

Reactivity:         Human, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey

Format:                 cFluor® BYG710¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. Please briefly centrifuge the reagent vial before use. Use appropriate personal protective equipment per the product safety data sheet when using this product.

Spectral signature of cFluor® BYG710 from a Cytek® Aurora 5 laser system equipped with 355, 405, 488, 561 and 640 nm lasers using CytekAssaySetting

REFERENCES

1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
2. Kern Petra S. et al. 1998. Immunity. 519-530
3. Veillette A. et al. 1988. Cell. 55:301-308

These products are for research use only. Not intended for use in diagnostic procedures.

¹cFluor® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.