The 8F1-1-4 monoclonal antibody binds human CD181, a 67-70 kD seven-transmembrane protein, also known as CXCR1, IL-8 receptor A (IL-8RA), and CDw128a. It is a CXC chemokine receptor that belongs to the G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR1 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:          8F1-1-4

Reactivity:       Human

Format:        cFluor^® BYG710¹

Isotype:              Mouse IgG2b, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1.  Rabinowich H, 1994. J Immunol. 152(2):517-526.
2. Aruffo A, et al. 1987. EMBO J. 6(11):3313-3316.
3. Lee DM, et al. 1996. Int Immunol. 8(8):1195-1203

For Research Use Only. Not for use in diagnostic or therapeutic procedures.