The IA6-2 monoclonal antibody reacts with the heavy chain of human Immunoglobulin D (IgD), a type I transmembrane glycoprotein and member of the immunoglobulin superfamily. IgD is co-expressed with membrane IgM on the surface of mature naïve B cells, where both isotypes associate with the Igα/Igβ (CD79a/CD79b) signaling heterodimer to form the B cell antigen receptor (BCR) complex. Engagement of the IgD-BCR by antigen mediates signal transduction that fine-tunes B cell activation, selection, and tolerance, complementing rather than duplicating IgM-BCR signaling. IgD expression is lost following isotype class switching, and a soluble form of IgD circulates at low levels in serum, where it has also been implicated in mucosal innate immune defense through interactions with basophils and mast cells.

PRODUCT DETAILS

Clone: IA6-2

Specificity: IgD

Reactivity: Human

Format: cFluor^® BYG667

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Geisberger, R. et al. 2006. Immunology 118: 429–437.
2. Chen, K. and Cerutti, A. 2011. Curr. Opin. Immunol. 23: 345–352.
3. Gutzeit, C. et al. 2018. Eur. J. Immunol. 48: 1101–1113.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG667 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.