The O323 monoclonal antibody binds to human CD27 (TNFRSF7), which consists of a homodimer of two 50-55 kDa type I membrane protein. CD27 is a lymphocyte specific member of the TNF receptor superfamily.¹ CD27 is expressed on a subset of thymocytes, mature T cells.² The expression further upregulated in T cell activation.³ Subpopulations of B cells and NK also express CD27.^4,5 In T cell-B cell interaction, CD27 binds to CD70 to provide costimulation to result in T cell activation and B cell differentiation and proliferation.⁶ The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:              O323

Reactivity:          Human

Format:        cFluor^® BYG610¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Akiba H, et al. JBC. 273, 13353 (1998)
2. De Jong R, et al. Eur J Immunol. 22, 993 (1992)
3. Ahlers, JD et al. Blood 115, 1678 (2010)
4. Wu YC, et al. Front Immunol. 26, 2 (2011)
5. Hayakawa Y & Smyth MJ J Immunol 176, 1517 (2006)
6. Borst J, Hendriks J & Xiao Y. Curr Opin Immunol. 17, 275 (2005)

These products are for research use only. Not intended for use in diagnostic procedures.

¹cFluor^® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.