The ICRF44 monoclonal antibody reacts with human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b associated with integrin β2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

 PRODUCT DETAILS

Clone:          ICRF44

Reactivity:     Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Monkey

Format:        cFluor^® BYG610¹

Isotype:              Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

 

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

 

REFERENCES

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Marsik C, et al. 2003. Shock 20:493.
4. Charles N, et al. 2010. Nat. Med. 16:701.
5. Thurlow LR, et al. 2010. Infect. Immun. 128:1128.
6. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.

7. Wen T, et al. 2014. J Immunol. 192:5481.

¹ cFluor® BYG610 a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.