The mouse monoclonal antibody clone WT31 binds to a conformational epitope formed by the human alpha/beta T cell receptor (TCR αβ) in complex with the CD3 epsilon chain. The TCR αβ heterodimer is composed of a 44 kDa alpha chain and a 37 kDa beta chain, whose specificity is determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR αβ heterodimer associates non-covalently with the CD3 complex, which is composed of at least five polypeptides including CD3 gamma, delta, epsilon, and zeta chains. Together, the TCR/CD3 complex recognizes antigen-derived peptides bound to MHC molecules on antigen-presenting cells such as B cells, dendritic cells, and macrophages, leading to T cell activation, intracellular signaling, and cytokine production. The TCR αβ heterodimer is expressed on the majority of peripheral blood T cells, while a minor population of CD3+ T cells expresses the alternative gamma/delta TCR heterodimer and is not recognized by WT31. The WT31 antibody has been reported to induce proliferation of resting T cells and to inhibit cytotoxic T cell activity. Some anti-CD3 antibodies partially inhibit WT31 staining, and clone SK7 is recommended for use in two-color experiments with WT31.

PRODUCT DETAILS

Clone: WT31

Specificity: TCR a/b

Reactivity: Human

Format: cFluor^® BYG575

Isotype: Mouse IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Spits, H. et al. 1985. J. Immunol. 135: 1922–1928.
2. Weiss, A. and Littman, D.R. 1994. Cell. 76: 263–274.
3. Cantrell, D. 1996. Annu. Rev. Immunol. 14: 259–274.

For research use only. Not intended for use in diagnostic procedures.