The mouse monoclonal antibody clone SN8 binds to human CD79b, also known as Igβ (immunoglobulin beta). CD79b is a 37–47 kDa type I transmembrane glycoprotein that forms a heterodimer with CD79a (Igα) and associates noncovalently with membrane-bound immunoglobulin to constitute the B-cell receptor (BCR) complex. CD79b is expressed throughout most stages of B-cell development, beginning at the pre-B cell stage and continuing through mature peripheral B cells, but is absent on plasma cells. The CD79a/CD79b signaling heterodimer contains immunoreceptor tyrosine-based activation motifs (ITAMs) within their cytoplasmic domains, which are essential for antigen-dependent B-cell activation, intracellular signaling, and B-cell maturation. Clone SN8 recognizes human CD79b and is suitable for the identification and immunophenotypic analysis of B lymphocytes by flow cytometry. CD79b detection is commonly used in studies of normal B-cell differentiation as well as in the characterization of B-cell leukemias and lymphomas.

PRODUCT DETAILS

Clone: SN8

Specificity: CD79b

Reactivity: Human

Format: cFluor^® BYG575

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Mason DY, Cordell JL, et al. Blood. 1995;86(4):1453-1459.
2. Chu PG, Arber DA, et al. Appl Immunohistochem Mol Morphol. 2001;9(2):97-106.

For research use only. Not intended for use in diagnostic procedures.