The mouse monoclonal antibody clone EH12.2H7 binds to human CD279 (PD-1), an inhibitory checkpoint receptor expressed on activated T cells, B cells, NK cells, and subsets of myeloid cells. CD279 plays a central role in immune exhaustion, tolerance, and suppression during chronic antigen exposure and malignancy. Clone EH12.2H7 binds an extracellular epitope and is suited for flow cytometric profiling of T-cell exhaustion, immune checkpoint expression, and tumor-infiltrating lymphocyte characterization

PRODUCT DETAILS

Clone: EH12.2H7

Specificity: CD279 (PD-1)

Reactivity: Human, African Green Monkey, Baboon, Chimpanzee, Common Marmoset, Cynomolgus Monkey, Rhesus Macaque, Squirrel Monkey, Non-Human Primate

Format: cFluor^® BYG575

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Dong H, et al. 2002. Nat Med. 8:793–800.
2. Wherry EJ. 2011. Nat Immunol. 12:492–499.
3. Gros A, et al. 2014. J Clin Invest. 124:2246–2259.

For research use only. Not intended for use in diagnostic procedures.