The SK1 monoclonal antibody binds to human CD8a, a 32-34-kDa type I glycoprotein that forms either as an α/α homodimer α/β heterodimer with its isoform CD8b. CD8 is found on thymocytes, a subset of peripheral blood T cells, majority expressing α/β heterodimer, and NK cells, majority expressing α/α homodimer¹. CD8 plays a role in antigen recognition and class I MHC-restricted T cell development. CD8 functions are associated with two domains of CD8a. CD8a ectodomain binding to the α3 domain of MHC class I increases the adhesion between CD8+ T lymphocytes and target cells. The cytoplasmic tail binding to the tyrosine kinase p56^lck contributes to signal transduction and T cell activation^2,3. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  2H7

Reactivity:         Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Squirrel Monkey

Format:              cFluor® B675

Isotype:               Mouse IgG2b, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

Spectral signature of cFluor^® B675 from a Cytek^® Aurora 5 laser system equipped with 355, 405, 488, 561 and 640 nm lasers using CytekAssaySetting.

REFERENCES

1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
2. Kern Petra S. et al. 1998. Immunity. 519-530
3. Veillette A. et al. 1988. Cell. 55:301-308

These products are for research use only. Not intended for use in diagnostic procedures.