Description: The monoclonal antibody 10F7MN recognizes human glycophorin A (also known as CD235a). The antibody can see both the M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease.

Applications Reported: This 10F7MN antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 10F7MN antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.

Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.

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