PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: 2H7CD155

Format: Super Bright™ 436

Reactivity: Human

Application: Flow Cytometry

Tested Dilution: 5 µL (0.25 µg)/test

Concentration: 5 µL/Test

Storage: 4° C, store in dark, DO NOT FREEZE!

Formulation: PBS, pH 7.2, containing 0.09% sodium azide

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The monoclonal antibody 2H7CD155 recognizes CD155 also known as the poliovirus receptor. The CD155 protein is a member of the Ig superfamily in which 4 isoforms exist; only the alpha and delta forms are expressed on the membrane of monocytes, prostate cancer cells, as well as neurectodermal and monocytic tumor cells lines. In addition, CD155 is found during mid-gastrulation during embryonic development. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD226 and CD96 are ligands for CD155. Blocking of CD155 is under therapeutic investigation for prevention of variants of poliovirus.

Applications Reported: This 2H7CD155 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7CD155 antibody has been pre-diluted and tested by flow cytometric analysis of U937 cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.