PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a, kappa

Clonality: Monoclonal

Clone: OX42

Format: PE

Reactivity: Rt

Application: Flow Cytometry

Tested Dilution: 0.125 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4). CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.

The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages. Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation.

Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.