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C57Bl/6 splenocytes were stained with 0.25 ug Biotin Anti-Mouse CD4 (30-0041) (solid line) or 0.25 ug Biotin Rat IgG2b isotype control (dashed line), followed by Streptavidin PE.

Biotin Anti-Mouse CD4 (GK1.5)

種類 30-0041-U025
通常価格
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説明

TDS | SDS

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will "compete" for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

 

Recent Citations:

Brian BF 4th, Sauer ML, Greene JT, Senevirathne SE, Lindstedt AJ, Funk OL, Ruis BL, Ramirez LA, Auger JL, Swanson WL, Nunez MG, Moriarity BS, Lowell CA, Binstadt BA, Freedman TS. A dominant function of LynB kinase in preventing autoimmunity. Sci Adv. 2022 Apr 22;8(16):eabj5227. doi: 10.1126/sciadv.abj5227. Epub 2022 Apr 22. PMID: 35452291.

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Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.

» View on PubMed

Name Biotin Anti-Mouse CD4 (GK1.5)
Cat. No. 30-0041
Alternative Names L3T4, T7
Gene ID 12504
Clone GK1.5
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity Syrian Hamster
Format Biotin
Application Flow Cytometry