TDS | SDS

The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Recent Publications:

Alam MM, Yang D, Li XQ, Liu J, Back TC, Trivett A, Karim B, Barbut D, Zasloff M, Oppenheim JJ. Alpha synuclein, the culprit in Parkinson disease, is required for normal immune function. Cell Rep. 2022 Jan 11;38(2):110090. doi: 10.1016/j.celrep.2021.110090. PMID: 35021075.

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Clark NM, Martinez LM, Murdock S, deLigio JT, Olex AL, Effi C, Dozmorov MG, Bos PD. Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells. Cell Rep. 2020 Dec 8;33(10):108482. doi: 10.1016/j.celrep.2020.108482. PMID: 33296659.

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Wali S, Flores JR, Jaramillo AM, et al. Immune Modulation to Improve Survival of Viral Pneumonia in Mice.  Am J Respir Cell Mol Biol. 2020;10.1165/rcmb.2020-0241OC. doi:10.1165/rcmb.2020-0241OC

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584

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Weist BM, Kurd N, Boussier J, Chan SW and Robey EA. 2015. Nat Immunol. 16(6): 635-641. (Flow Cytometry)