The mouse monoclonal antibody clone L17F12 binds to human CD5, a 67 kDa single chain type I glycoprotein in the scavenger receptor family. CD5, also known as Leu-1, Ly-1, or T1, is expressed on thymocytes, T cells, and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction.

PRODUCT DETAILS

Clone: L17F12

Specificity: CD5

Reactivity: Human

Format: APC-Cyanine7

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Challagundla P, Jorgensen JL, Kanagal-Shamanna R, et al. Archives of Pathology & Laboratory Medicine. 2014;138(7):903–909.
2. Friedman D, Mehtani DP, Vidler JB, Patten PEM, Hoogeboom R. bioRxiv 2023.12.27.573410
3. Kaplan D, Smith D, Meyerson, H. Proc. Natl. Acad. Sci. U.S.A. 2001;98(24):13850-13853.

For research use only. Not intended for use in diagnostic procedures.

APC-Cyanine7 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.