The mouse monoclonal antibody clone AK2 binds to human CD42b (GPIb alpha), a membrane glycoprotein with a molecular weight of 135-145 kDa. This protein is expressed on the surface of platelets and megakaryocytes, serving as an important marker for platelet activation. CD42b plays a crucial role in coagulation and the formation of blood clots by being a part of the GPIb-IX-V receptor complex, which helps attach platelets to surfaces that promote blood clotting. CD42b interacts with von Willebrand factor (VWF), thrombin, and several coagulation factors, and its extracellular domain can also influence metastasis. It is primarily found on platelets and megakaryocytes in the bone marrow. Additionally, various polymorphisms and mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.

PRODUCT DETAILS

Clone: AK2

Specificity: CD42b

Reactivity: Human

Format: APC

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Ruggeri, Z.M. 1997. J. Clin. Invest. 99: 559–564.
2. Lenting, P.J. et al. 2015. Cell. Mol. Life Sci. 72: 187–204.
3. Rauch, A., et al. 2022. J. Thromb. Haemost. 20(9): 2058–2069..

For research use only. Not intended for use in diagnostic procedures.