The mouse monoclonal antibody clone 29E.2A3 binds to human CD274, also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. This binding plays a role in clonal selection, elimination of autoreactive lymphocytes, and tolerance development. Additionally, PD-L1 can limit the activation of mature T cells. As part of the B7 ligand family, its interactions with the CD28 receptor family, including CTLA-4, are crucial for balancing T cell activation and tolerance. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

PRODUCT DETAILS

Clone: 29E.2A3

Specificity: CD274 (PD-L1, B7-H1)

Reactivity: Human

Format: Alexa Fluor™ 700

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Latchman et al. 2001. Nat Immunol. 2:261-8.

For research use only. Not intended for use in diagnostic procedures.