The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).

PRODUCT DETAILS

Clone: 24F.10C12

Specificity: CD273 (PD-L2, B7-DC)

Reactivity: Human

Format: Alexa Fluor™ 647

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Green MR, et al. 2010. Blood 116:3268-77.
2. Rodríguez-García M, et al. 2011. J Leukoc Biol 89:507-15.
3. Semnani RT, et al. 2011. Infect Immun 79:3957-65.

For research use only. Not intended for use in diagnostic procedures.