The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.

PRODUCT DETAILS

Clone: TP25.99SF

Specificity: MHC Class I (HLA-ABCE)

Reactivity: Human

Format: Alexa Fluor™ 488

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Desai SA, et al. 2000. J Immunol 165:3275-83.
2. Perosa F, et al. 2003. J Immunol 171:1918-26.
3. Moy FJ, et al. 2000. J Biol Chem 275:24679-85.
4. Paul P, et al. 2000. Hum Immunol 61:1177-95.

For research use only. Not intended for use in diagnostic procedures.