PRODUCT DETAILS

Host: Mouse

Isotype: IgM, kappa

Clonality: Monoclonal

Clone: eBio3G11 (SM3G11, 3G11)

Format: eFluor 660

Reactivity: Ms, Rt

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naive and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3 epsilon, suggesting close proximity of these two proteins.

Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.

Applications Reported: This eBio3G11 (SM3G11, 3G11) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio3G11 (SM3G11, 3G11) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.