The mouse monoclonal antibody clone 4H11 binds to human CD34, also known as mucosialin. CD34 belongs to a protein family, which also includes endoglycan and podocalyxin. Members of this family are single-pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. Despite this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. The 4H11 clone belongs to class III, indicating that it reacts with a protein epitope on CD34.

PRODUCT DETAILS

Clone: 4H11

Specificity: CD34

Reactivity: Human

Format: cFluor^® BYG710^*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Schlossman SF, et al. 1995. Leukocyte Typing V: White Cell Differentiation Antigen.
2. Felschow DM, et al. 2001. Blood 97:3768.
3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97.

For research use only. Not intended for use in diagnostic procedures.

^* cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.