The mouse monoclonal antibody clone SK7 binds to human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR) and the CD3 zeta chain to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex recognizes antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines, or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The SK7 antibody can be used as a phenotypic marker for human T cells. An alternative Anti-Human CD3 clone, OKT3, can block the binding of clone SK7.

PRODUCT DETAILS

Clone: SK7

Specificity: CD3

Reactivity: Human, Chimpanzee

Format: cFluor^® V610

Isotype: IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.