The mouse monoclonal antibody clone 104D2 binds to human CD117 (also known as c-Kit), a 145 kDa type 1 transmembrane receptor that serves as the receptor for Stem Cell Factor (SCF). CD117 is expressed on hematopoietic stem cells in the bone marrow, including progenitor cells and mast cells, and is used as a flow marker to identify hematopoietic progenitor cells, mast cells, melanocytes, and gastrointestinal stromal tumor cells (GISTs). It constitutes about 1-4% of bone marrow cells, with 50-70% co-expressing CD34. In contrast, B-lymphoid committed progenitor cells (CD34+ CD19+) have low levels of CD117. Among CD34- bone marrow cells, only a few cells, primarily erythroid cells, express the CD117 receptor. CD117/c-Kit is a tyrosine kinase receptor. Upon binding of SCF, CD117/c-Kit undergoes dimerization and autophosphorylation, activating crucial signaling pathways for hematopoietic stem cell proliferation and differentiation. The antibody reportedly does not interfere with the binding of c-Kit ligand or the SR1 antibody.

PRODUCT DETAILS

Clone: 104D2

Specificity: CD117 (c-Kit)

Reactivity: Human, Rhesus Macaque, Cynomolgus Monkey

Format: cFluor^® BYG610

Isotype: IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Briddell RA, et al, 1992. Blood. 79:3159-3167.
2. Broudy, V.C. et al. 1998. Blood. 91 (3): 898-906.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG610 is equivalent to RPE-CF^®596R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.