Ghost Dye^® Blue 516 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye^® Blue 516 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

PRODUCT DETAILS

Excitation Laser: Blue (488 nm)

Emission: 516

Format: Ghost Dye^® Blue 516, Viability Dye

Application: Flow Cytometry

Storage: -20°C protected from light and moisture

Formulation: 1 µL/test in DMSO

Data Sheets: TDS | SDS

Protocol: Download Protocol

PREPARATION & STORAGE
Ghost Dye^® Blue 516 is provided in solution prepared in anhydrous DMSO and should be protected from light and moisture. Store vial at -20°C. Prior to use, allow vial to equilibrate to room temperature before opening. Ghost Dye^® Blue 516 is stable through 20 freeze/thaw cycles, if needed, aliquot smaller volumes and store at -20°C.

APPLICATION NOTES
Ghost Dye^® Blue 516 has been quality-tested for flow cytometry using mouse bone marrow cells and can be used at 1 µL/mL of cell suspension. The concentration required for optimal performance should be determined empirically by investigator

Ghost Dye^® Blue 516 is excited by the Blue (488 nm) laser line and has a peak emission of 516 nm that can be detected using a 530/30 band pass filter commonly used for detection of FITC.

For Research Use Only. Not intended for use in diagnostic procedures.

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