The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

PRODUCT DETAILS

Clone: 6D2.1

Specificity: PKAc

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: WB

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).

REFERENCES

1. Doucas V, et al. 2000. Proc Natl Acad Sci U S A 97:11893-8.
2. Ferraris JD, et al. 2002. Proc Natl Acad Sci U S A 99:16800-5.
3. Komagiri Y, et al. 2007. Biomed Res 28:177-89.
4. Kiger JA Jr, et al. 1999. Genetics 152:281-90.
5. Beebe SJ, et al. 1990. Mol Endocrinol 4:465-75.

For research use only. Not intended for use in diagnostic procedures.