The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

PRODUCT DETAILS

Clone: MEM-255

Specificity: PAG1

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: WB, IHC-P, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 2 μg/ml. Intracellular staining.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil, spleen.
Western blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.

REFERENCES

1. Semac I, et al. 2003. Cancer Res 63:534-40.
2. Brdicka T, et al. 2000. J Exp Med 191:1591-604.
3. Brdicková N, et al. 2001. FEBS Lett 507:133-6.
4. Schatzlmaier P, et al. 2015. Sci Signal 8:rs11.
5. Tedoldi S, et al. 2006. Blood 107:213-21.
6. Davidson D, et al. 2003. Mol Cell Biol 23:2017-28.

For research use only. Not intended for use in diagnostic procedures.