The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

PRODUCT DETAILS

Clone: MEM-147

Specificity: MHC Class I (HLA-ABC)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: FC, IP, WB

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1-5 μg/ml.
Western blotting: Non-reducing conditions.

REFERENCES

1. Ilangumaran S, et al. 1998. Blood 91:3901-8.
2. Drbal K, et al. 2007. Int Immunol 19:675-84.
3. Tran TM, et al. 2001. Immunogenetics 53:440-6.

For research use only. Not intended for use in diagnostic procedures.